Tha am pasgan seo air a chleachdadh airson lorg searbhag niuclasach càileachdail in vitro de Coronavirus 2019-Novel (2019-nCoV), Influenza A agus Influenza B aig an aon àm ann an sampallan analach a’ toirt a-steach swaban oropharyngeal agus sputum.Tha seataichean primer agus probes le bileagan FAM air an dealbhadh airson lorg sònraichte air an dà chuid ginean ORFlab agus N de 2019-nCoV, probe le bileag VIC airson gine RNase P daonna mar smachd a-staigh, probe le bileag ROX airson gine M de Influenza A, probe le bileagan CY5 airson gine NS den Influenza B.
| Co-phàirtean | 50 deuchainn / pasgan |
| Bufair freagairt RT-PCR | 915 μL × 1 tiùb |
| RT-PCR measgachadh enzyme | 75 μL × 1 tiùb |
| Smachd adhartach | 100 μL × 1 tiùb |
| Smachd àicheil | 100 μL × 1 tiùb |
Cugallachd: 200 leth-bhreac / ml.
Sònrachadh: Sònrachadh: Gun ath-bhualadh le SARS-CoV, MERS-CoV, CoV-HKU1, CoV-OC43, CoV-229E, CoV-NL63, Bhìoras parainfluenza, adenovirus daonna, rhinovirus daonna, bhìoras syncytial analach (A), Syncytial analach Bhìoras (B), Enterovirus A, Staphylococcus Aureus, Streptococcus Pneumonia, Klebsiella Pneumonia.
mionaideachd: CV ≤ 5%.
Siostam PCR fìor-ùine:Aehealth Diagenex AL,Molarray MA-6000, ABI 7500, ViiATM7, QuantStudio 7 flex, Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000 / Q, Bio-Rad CFX96 TouchTM/iQTM5, SLAN-96S/96P